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Correlation between MCP-1 + podocyte-derived uEVs and clinical parameters . Spearman correlation analysis shows that the proportion of MCP-1 + PODXL + (red) and MCP-1 + PODXL + NEPHRIN + (black) uEVs were both directly correlated with (A) HOMA-IR, (B) insulin, and urinary (U) levels of (C) KIM-1, (D) NGAL, and (E) urinary protein. A trend line is shown to illustrate the overall directionality of the association. MCP-1, monocyte chemoattractant protein-1; PODXL, podocalyxin; NEPHRIN, nephrin; HOMA-IR, homoeostatic model assessment for insulin resistance; KIM-1, kidney injury <t>molecule-1;</t> NGAL, neutrophil gelatinase-associated lipocalin.
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Correlation between MCP-1 + podocyte-derived uEVs and clinical parameters . Spearman correlation analysis shows that the proportion of MCP-1 + PODXL + (red) and MCP-1 + PODXL + NEPHRIN + (black) uEVs were both directly correlated with (A) HOMA-IR, (B) insulin, and urinary (U) levels of (C) KIM-1, (D) NGAL, and (E) urinary protein. A trend line is shown to illustrate the overall directionality of the association. MCP-1, monocyte chemoattractant protein-1; PODXL, podocalyxin; NEPHRIN, nephrin; HOMA-IR, homoeostatic model assessment for insulin resistance; KIM-1, kidney injury <t>molecule-1;</t> NGAL, neutrophil gelatinase-associated lipocalin.
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Correlation between MCP-1 + podocyte-derived uEVs and clinical parameters . Spearman correlation analysis shows that the proportion of MCP-1 + PODXL + (red) and MCP-1 + PODXL + NEPHRIN + (black) uEVs were both directly correlated with (A) HOMA-IR, (B) insulin, and urinary (U) levels of (C) KIM-1, (D) NGAL, and (E) urinary protein. A trend line is shown to illustrate the overall directionality of the association. MCP-1, monocyte chemoattractant protein-1; PODXL, podocalyxin; NEPHRIN, nephrin; HOMA-IR, homoeostatic model assessment for insulin resistance; KIM-1, kidney injury <t>molecule-1;</t> NGAL, neutrophil gelatinase-associated lipocalin.
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Correlation between MCP-1 + podocyte-derived uEVs and clinical parameters . Spearman correlation analysis shows that the proportion of MCP-1 + PODXL + (red) and MCP-1 + PODXL + NEPHRIN + (black) uEVs were both directly correlated with (A) HOMA-IR, (B) insulin, and urinary (U) levels of (C) KIM-1, (D) NGAL, and (E) urinary protein. A trend line is shown to illustrate the overall directionality of the association. MCP-1, monocyte chemoattractant protein-1; PODXL, podocalyxin; NEPHRIN, nephrin; HOMA-IR, homoeostatic model assessment for insulin resistance; KIM-1, kidney injury <t>molecule-1;</t> NGAL, neutrophil gelatinase-associated lipocalin.
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Correlation between MCP-1 + podocyte-derived uEVs and clinical parameters . Spearman correlation analysis shows that the proportion of MCP-1 + PODXL + (red) and MCP-1 + PODXL + NEPHRIN + (black) uEVs were both directly correlated with (A) HOMA-IR, (B) insulin, and urinary (U) levels of (C) KIM-1, (D) NGAL, and (E) urinary protein. A trend line is shown to illustrate the overall directionality of the association. MCP-1, monocyte chemoattractant protein-1; PODXL, podocalyxin; NEPHRIN, nephrin; HOMA-IR, homoeostatic model assessment for insulin resistance; KIM-1, kidney injury <t>molecule-1;</t> NGAL, neutrophil gelatinase-associated lipocalin.
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Correlation between MCP-1 + podocyte-derived uEVs and clinical parameters . Spearman correlation analysis shows that the proportion of MCP-1 + PODXL + (red) and MCP-1 + PODXL + NEPHRIN + (black) uEVs were both directly correlated with (A) HOMA-IR, (B) insulin, and urinary (U) levels of (C) KIM-1, (D) NGAL, and (E) urinary protein. A trend line is shown to illustrate the overall directionality of the association. MCP-1, monocyte chemoattractant protein-1; PODXL, podocalyxin; NEPHRIN, nephrin; HOMA-IR, homoeostatic model assessment for insulin resistance; KIM-1, kidney injury <t>molecule-1;</t> NGAL, neutrophil gelatinase-associated lipocalin.
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Correlation between MCP-1 + podocyte-derived uEVs and clinical parameters . Spearman correlation analysis shows that the proportion of MCP-1 + PODXL + (red) and MCP-1 + PODXL + NEPHRIN + (black) uEVs were both directly correlated with (A) HOMA-IR, (B) insulin, and urinary (U) levels of (C) KIM-1, (D) NGAL, and (E) urinary protein. A trend line is shown to illustrate the overall directionality of the association. MCP-1, monocyte chemoattractant protein-1; PODXL, podocalyxin; NEPHRIN, nephrin; HOMA-IR, homoeostatic model assessment for insulin resistance; KIM-1, kidney injury molecule-1; NGAL, neutrophil gelatinase-associated lipocalin.

Journal: eBioMedicine

Article Title: Renal podocyte senescence in human obesity detected by urinary extracellular vesicles

doi: 10.1016/j.ebiom.2025.106116

Figure Lengend Snippet: Correlation between MCP-1 + podocyte-derived uEVs and clinical parameters . Spearman correlation analysis shows that the proportion of MCP-1 + PODXL + (red) and MCP-1 + PODXL + NEPHRIN + (black) uEVs were both directly correlated with (A) HOMA-IR, (B) insulin, and urinary (U) levels of (C) KIM-1, (D) NGAL, and (E) urinary protein. A trend line is shown to illustrate the overall directionality of the association. MCP-1, monocyte chemoattractant protein-1; PODXL, podocalyxin; NEPHRIN, nephrin; HOMA-IR, homoeostatic model assessment for insulin resistance; KIM-1, kidney injury molecule-1; NGAL, neutrophil gelatinase-associated lipocalin.

Article Snippet: Additional biomarkers were quantified using commercially available enzyme-linked immunosorbent assays, including urinary neutrophil gelatinase-associated lipocalin (NGAL; ThermoFisher, Cat#KIT036), kidney injury molecule-1 (KIM-1; R&D Systems, Minneapolis, MN, Cat#DKM100), insulin (Sigma–Aldrich, Saint Louis, MO, Cat#RAB0327), tumour necrosis factor-alpha (TNF-α; Sigma–Aldrich, Cat#RAB0476), and monocyte chemoattractant protein-1 (MCP-1; R&D, Cat#DCP00).

Techniques: Derivative Assay

Correlation between P16 + MCP-1 + PODXL + podocyte-derived uEVs and clinical parameters . The proportion of P16 + MCP-1 + PODXL + uEVs in HV (blue, n = 10) and OB (red, n = 21) was significantly positively correlated with (A) BMI, (B) HOMA-IR, (C) insulin, (D) and urinary (U) levels of KIM-1, (E) NGAL, (F) protein, and (G) TNF-α; BMI, body mass index; HOMA-IR, homoeostatic model assessment for insulin resistance; A trend line is shown to illustrate the overall directionality of the association. KIM-1, kidney injury molecule-1; NGAL, neutrophil gelatinase-associated lipocalin; TNF-α, tumour necrosis factor-alpha.

Journal: eBioMedicine

Article Title: Renal podocyte senescence in human obesity detected by urinary extracellular vesicles

doi: 10.1016/j.ebiom.2025.106116

Figure Lengend Snippet: Correlation between P16 + MCP-1 + PODXL + podocyte-derived uEVs and clinical parameters . The proportion of P16 + MCP-1 + PODXL + uEVs in HV (blue, n = 10) and OB (red, n = 21) was significantly positively correlated with (A) BMI, (B) HOMA-IR, (C) insulin, (D) and urinary (U) levels of KIM-1, (E) NGAL, (F) protein, and (G) TNF-α; BMI, body mass index; HOMA-IR, homoeostatic model assessment for insulin resistance; A trend line is shown to illustrate the overall directionality of the association. KIM-1, kidney injury molecule-1; NGAL, neutrophil gelatinase-associated lipocalin; TNF-α, tumour necrosis factor-alpha.

Article Snippet: Additional biomarkers were quantified using commercially available enzyme-linked immunosorbent assays, including urinary neutrophil gelatinase-associated lipocalin (NGAL; ThermoFisher, Cat#KIT036), kidney injury molecule-1 (KIM-1; R&D Systems, Minneapolis, MN, Cat#DKM100), insulin (Sigma–Aldrich, Saint Louis, MO, Cat#RAB0327), tumour necrosis factor-alpha (TNF-α; Sigma–Aldrich, Cat#RAB0476), and monocyte chemoattractant protein-1 (MCP-1; R&D, Cat#DCP00).

Techniques: Derivative Assay